In vitro degradation rate of concentrated growth factors in simulated body fluid and simulated saliva fluid

doi:10.3969/j.issn.2095-4344.0716

Abstract

Abstract:

BACKGROUND: Bioabsorbable biomaterials are of crucial importance in tissue engineering applications, and various factors affect their degradation.

OBJECTIVE: To compare the degradation characteristics of concentrated growth factor (CGF) clot and CGF membrane in simulated body fluid (SBF) and simulated saliva fluid (SSF).

METHODS: Fifteen volunteers were selected, and human blood samples were collected for the preparation of CGF clot or CGF membrane. All specimens from each subject were averagely divided into four groups: group A, CGF clot in SBF; group B, CGF clot in SSF; group C, CGF membrane in SBF; group D, CGF membrane in SSF. The specimens were subjected to the immersion test. The average daily rate of degradation of each group was calculated after the samples were thoroughly degraded, and weight loss ratio per unit time was also determined.

RESULTS AND CONCLUSION: (1) The mean degradation time in groups A-D were (14.0±0.7), (9.7±0.9), (9.9±1.2) and (7.2±0.7) days, respectively. (2) By comparing CGF membrane with CGF clot in the same simulated fluid, the average daily degradation rate of CGF clot (groups A, B) was statistically significantly lower than counterparts of CGF membrane (groups C, D) (P < 0.05). By comparison between SBF and SSF, the average daily degradation rate in the SBF (groups A, B) was significantly lower than counterparts in the SSF (groups C, D) (P < 0.05). Overall, the degradation rate of CGF membrane is higher than that of CGF clot under the same degradation environment; for CGF membrane or CGF clot, the degradation rate in SSF is higher than that in SBF.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Guided Tissue Regeneration, Saliva, Blood Platelets, Fibrin, Tissue Engineering

CLC Number:

R318

Zou Xin-ming, Huang Na, Wang Yuan-qin, Li Shao-bing, Tang You-chao. In vitro degradation rate of concentrated growth factors in simulated body fluid and simulated saliva fluid[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(10): 1559-1564.

Figures/Tables 4

References

[1]Buser D,Sennerby L,De Bruyn H.Modern implant dentistry based on osseointegration: 50 years of progress, current trends and open questions.Periodontol 2000.2017;73(1):7-21.

[2]Bottino MC,Thomas V,Schmidt G,et al.Recent advances in the development of GTR/GBR membranes for periodontal regeneration--a materials perspective.Dent Mater. 2012;28(7): 703-721.

[3]Rakhmatia YD,Ayukawa Y,Furuhashi A,et al.Current barrier membranes: titanium mesh and other membranes for guided bone regeneration in dental applications.J Prosthodont Res. 2013;57(1):3-14.

[4]Misch C.Vertical alveolar ridge augmentation in implant dentistry: A surgical manual. LWW,2017.

[5]Misch CM.Horizontal alveolar ridge augmentation in implant dentistry: A surgical manual.LWW,2017.

[6]Simonpieri A,Del Corso M,Vervelle A,et al.Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 2: Bone graft, implant and reconstructive surgery.Curr Pharm Biotechnol.2012;13(7):1231-1256.

[7]Del Corso M,Vervelle A,Simonpieri A,et al.Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 1: Periodontal and dentoalveolar surgery.Curr Pharm Biotechnol. 2012;13(7):1207-1230.

[8]Rodella LF,Bonazza V.Platelet preparations in dentistry: Why, where, when. World J Stomatol.2015;4: 39-55.

[9]Marx RE.Platelet-rich plasma (prp): What is prp and what is not prp? Implant Dent.2001;10:225-228.

[10]Dohan DM,Choukroun J,Diss A,et al.Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):e37-44.

[11]Dohan DM,Choukroun J,Diss A,et al.Platelet-rich fibrin (prf): A second-generation platelet concentrate. Part ii: Platelet-related biologic features.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):e45-50.

[12]Bonazza V,Dellavia C,Labanca M,et al.Concentrated growth factors (cgf): Morphological and biochemical characterization. Ital J Anat Embryol.2015;120:18.

[13]Honda H,Tamai N,Naka N,et al.Bone tissue engineering with bone marrow-derived stromal cells integrated with concentrated growth factor in rattus norvegicus calvaria defect model.J Artif Organs. 2013;16(3):305-315.

[14]Rodella LF,Favero G,Boninsegna R,et al.Growth factors, cd34 positive cells, and fibrin network analysis in concentrated growth factors fraction.Microsc Res Tech.2011;74(8):772-777.

[15]黄娜,吴锋,李平,等.应用浓缩生长因子行上颌窦内提升后种植体周围骨变化[J].中国组织工程研究,2015, 19(47):7575-7582.

[16]Bozkurt Do?an ?,Öngöz Dede F,Ball? U,et al.Concentrated growth factor in the treatment of adjacent multiple gingival recessions: A split‐mouth randomized clinical trial.J Clin Periodontol. 2015; 42(9):868-875.

[17]Chen Y,Cai Z,Zheng D,et al.Inlay osteotome sinus floor elevation with concentrated growth factor application and simultaneous short implant placement in severely atrophic maxilla. Sci Rep. 2016;6:27348.

[18]Kim TH,Kim SH,Sándor GK,et al.Comparison of platelet-rich plasma (prp), platelet-rich fibrin (prf), and concentrated growth factor (cgf) in rabbit-skull defect healing.Arch Oral Biol. 2014;59: 550-558.

[19]程扬,刘敏.浓缩生长因子在上颌窦侧壁开窗窦底提升即刻种植中的成骨效果:单中心、随机对照临床试验方案[J]. 中国组织工程研究, 2017,21(4):574-579.

[20]Ying X,Chen Y,Luo N,et al.The use of concentrated growth factors and autogenous bone for periodontal soft tissue augmentation and bone regeneration: A case report.Elect J Biol. 2017;13(3): 269-273.

[21]李永斌,孙迎春,韦荣智,等.浓缩生长因子纤维蛋白与富血小板纤维蛋白体外降解的对比[J].中国组织工程研究, 2017,21(14): 2234-2240.

[22]Isobe K,Watanebe T,Kawabata H,et al.Mechanical and degradation properties of advanced platelet-rich fibrin (a-prf), concentrated growth factors (cgf), and platelet-poor plasma-derived fibrin (pptf). Int J Implant Dent.2017;3(1):17.

[23]Oyane A,Kim HM,Furuya T,et al.Preparation and assessment of revised simulated body fluids.J Biomed Mater Res A. 2003;65(2): 188-195.

[24]Qiu KJ,Lin WJ,Zhou FY,et al.Ti-Ga binary alloys developed as potential dental materials.Mater Sci Eng C Mater Biol Appl.2014; 34:474-483.

[25]肖悦.人工口腔模拟的口腔环境条件[J].国外医学:口腔医学分册, 2001,28(3):146-148.

[26]Park HC,Kim SG,Oh JS,et al.Early bone formation at a femur defect using cgf and prf grafts in adult dogs: A comparative study. Implant Dent.2016;25:387-393.

[27]Ohyama Y,Tanaka T,Shimizu T,et al.Runx2/smad3 complex negatively regulates tgf-β-induced connective tissue growth factor gene expression in vascular smooth muscle cells.J Atheroscler Thromb. 2012;19(1):23-35.

[28]Krause DS,Fulzele K,Catic A,et al.Differential regulation of myeloid leukemias by the bone marrow microenvironment.Nat Med.2013;19(11):1513-1517.

[29]Mezawa M,Araki S,Takai H,et al.Regulation of human bone sialoprotein gene transcription by platelet-derived growth factor-bb. Gene.2009;435:80-87.

[30]Xiao WD,Zhong ZM,Tang YZ,et al.Repair of critical size bone defects with porous poly(D,L-lactide)/nacre nanocomposite hollow scaffold.Saudi Med J.2012;33(6):601-607.

[31]Sheng MH,Lau KH,Baylink DJ.Role of Osteocyte-derived Insulin-Like Growth Factor I in Developmental Growth, Modeling, Remodeling, and Regeneration of the Bone.J Bone Metab. 2014; 21(1):41-54.

[32]Chen F,Pan S,Feng H.Distribution and content of transforming growth factor-β1 and vascular endothelial growth factor in each layer of concentrated growth factors.Beijing Da Xue Xue Bao Yi Xue Ban. 2016;48(5):860-865.

[33]Bonazza V,Borsani E,Buffoli B,et al.In vitro treatment with concentrated growth factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines.Cell Biol Int. 2017. doi: 10.1002/cbin.10908. [Epub ahead of print]

[34]Bonazza V,Boninsegna R,Merigo D,et al.In vitro effects of concentrated growth factors on bmp-2 synthesis by human osteoblasts.Italian J Anat Embryol.2016;121:73.

[35]Chen G,Deng C,Li YP.TGF-β and BMP signaling in osteoblast differentiation and bone formation.Int J Biol Sci. 2012;8(2): 272-288.

[36]De la Riva B,Sánchez E,Hernández A,et al.Local controlled release of vegf and pdgf from a combined brushite–chitosan system enhances bone regeneration.J Control Release. 2010; 143(1):45-52.

[37]Zelzer E,Glotzer DJ,Hartmann C,et al.Tissue specific regulation of vegf expression during bone development requires cbfa1/runx2. Mech Dev.2001;106(1-2):97-106.

[38]Starup-Linde J,Vestergaard P.Biochemical bone turnover markers in diabetes mellitus-a systematic review.Bone.2016;82:69-78.

[39]Dohan Ehrenfest DM,Bielecki T,Jimbo R,et al.Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (p-prp) gel and a leukocyte-and platelet-rich fibrin (l-prf).Curr Pharm Biotechnol. 2012;13(7):1145-1152.

[40]马西.纤维蛋白形成与降解[J].微循环学杂志,2002,12(4):42-44.

[41]白彭,叶平,吴润发,等.两种胶原膜暴露于口腔环境中降解作用的对照研究[J].中国口腔种植学杂志,2011,16(1): 56-57.